Journal: Journal of Lipid Research

Article Title: Inhibition of ABCA1 protein degradation promotes HDL cholesterol efflux capacity and RCT and reduces atherosclerosis in mice [S]

doi: 10.1194/jlr.M054742

Figure Lengend Snippet: IMM-H007 increases circulating HDL cholesterol levels and facilitates cholesterol excretion via feces. Animals (n = 8–12/group) were fed a control chow or Paigen diet and treated with vehicle, probucol (200 mg/kg), or the indicated doses of IMM-H007. A: Plasma HDL levels of Paigen diet-fed apoE−/− mice treated for 6 weeks. B: Plasma HDL levels of Paigen diet-fed WT mice treated with IMM-H007 for 2 weeks. C. Plasma HDL levels of vehicle- and IMM-H007-treated hamsters fed a Paigen diet for 2 weeks. D. FPLC lipoprotein profiles from pooled plasma (n = 8) of vehicle- or IMM-H007-treated WT mice fed a chow diet for 2 weeks. E, F: Fecal neutral sterol secretion of chow diet-fed (E) and Paigen diet-fed (F) mice treated for 2 weeks. Data are mean ± SEM. *P < 0.05, **P < 0.01 versus control.

Article Snippet: Plasma lipid profiles Plasma HDL levels were measured by cholesterol enzymatic kit (BioSino Corporation) after precipitation of apoB-containing lipoproteins by adding 100 μl plasma to 40 μl 20% PEG 8000 (P-2139 in 200 mmol/l glycine, pH 10; Sigma-Aldrich) solution ( 21 , 22 ).

Techniques: Control, Clinical Proteomics

Journal: Journal of Lipid Research

Article Title: Inhibition of ABCA1 protein degradation promotes HDL cholesterol efflux capacity and RCT and reduces atherosclerosis in mice [S]

doi: 10.1194/jlr.M054742

Figure Lengend Snippet: IMM-H007 promotes cholesterol efflux in vitro via ABCA1. J774 cells (A) and THP-1 cells (B) were used to evaluate cholesterol efflux to apoB-depleted serum (PEG-HDL) from vehicle- or IMM-H007-treated mice. ABCA1-mediated efflux was upregulated with 0.3 mM cAMP in J774 cells and with 10 μM 9-cis-retinoic acid in THP-1 cells, and it was determined as the proportion of the cholesterol inhibited by 20 μM probucol. In J774 cells, SR-BI-mediated efflux was determined as the proportion of cholesterol efflux inhibited by 1 μM BLT-1. Other modes of efflux were calculated as total efflux minus ABCA1- and SR-BI-mediated efflux. Plasma from vehicle- and IMM-H007-treated chow diet-fed WT mice (n = 8) was obtained 2 weeks after treatment; PEG-HDL was prepared by removal of apoB lipoproteins with polyethylene glycol and diluted to 2.5% in medium as the efflux acceptor. C, D: ABCA1-mediated efflux was defined as the percent cholesterol efflux to apoA-I (10 μg/ml, 4 h incubation) from vehicle- versus IMM-H007-treated cells that were also treated with cAMP [J774 cells (C)] or 9-cis-retinoic acid [THP-1 cells (D)]. E: SR-BI-mediated efflux from vehicle- versus IMM-H007-treated J774 cells by PEG-HDL isolated from vehicle-treated mice was defined as percent cholesterol efflux from cells treated with cAMP minus the percent cholesterol efflux from comparably treated cells additionally preincubated with BLT-1 (1 μM) for 2 h. F: Aqueous diffusion-mediated cholesterol efflux to methyl-β-cyclodextrin (1 mg/ml, 30 min incubation) from vehicle- versus IMM-H007-treated J774 cells. Data are mean ± SEM, n = 3. *P < 0.05, **P < 0.01 versus control. NS, nonsignificant.

Article Snippet: Plasma lipid profiles Plasma HDL levels were measured by cholesterol enzymatic kit (BioSino Corporation) after precipitation of apoB-containing lipoproteins by adding 100 μl plasma to 40 μl 20% PEG 8000 (P-2139 in 200 mmol/l glycine, pH 10; Sigma-Aldrich) solution ( 21 , 22 ).

Techniques: In Vitro, Clinical Proteomics, Incubation, Isolation, Diffusion-based Assay, Control

Journal: Journal of Lipid Research

Article Title: Inhibition of ABCA1 protein degradation promotes HDL cholesterol efflux capacity and RCT and reduces atherosclerosis in mice [S]

doi: 10.1194/jlr.M054742

Figure Lengend Snippet: IMM-H007 enhances HDL-mediated RCT in vivo. 3H-cholesterol and acLDL-loaded J774 macrophages were injected into apoE−/− mice fed a Paigen diet and treated with vehicle, probucol (200 mg/kg), or the indicated doses of IMM-H007 for 2 weeks (n = 5), and the amount of 3H-tracer in plasma (A), bile acid (B), liver (C), and feces (D) was determined by scintillation counting. Data are mean ± SEM, except in (A), in which legibility has been optimized by showing only mean values for 50 and 100 mg/kg IMM-H007. In those groups, all SEMs were less than 25% of mean values. *P < 0.05, **P < 0.01 versus control.

Article Snippet: Plasma lipid profiles Plasma HDL levels were measured by cholesterol enzymatic kit (BioSino Corporation) after precipitation of apoB-containing lipoproteins by adding 100 μl plasma to 40 μl 20% PEG 8000 (P-2139 in 200 mmol/l glycine, pH 10; Sigma-Aldrich) solution ( 21 , 22 ).

Techniques: In Vivo, Injection, Clinical Proteomics, Control

Journal: Scientific Reports

Article Title: Dietary supplementation with hybrid palm oil alters liver function in the common Marmoset

doi: 10.1038/s41598-018-21151-0

Figure Lengend Snippet: Biochemical parameters analyzed during the nutritional intervention (n = 10 animals per group). AP: African Palm Group; HP: Hybrid Palm Group. Glucose, Total cholesterol, HDL cholesterol, LDL cholesterol, VLDL cholesterol, and triglycerides were determined with standard colorimetric methods. Values expressed as mean ± SEM. **Indicates a statistical difference at p < 0.02.

Article Snippet: Fractions of 0.4 ml were collected and cholesterol and triglyceride concentrations in each fraction were determined enzymatically (Centronic GmbH, Wartenberg, Germany).

Techniques:

Journal: BioMed Research International

Article Title: A High-Fat Diet Exacerbates the Course of Experimental Trypanosoma cruzi Infection That Can Be Mitigated by Treatment with Simvastatin

doi: 10.1155/2020/1230461

Figure Lengend Snippet: Trypanosoma cruzi increases total cholesterol, LDL, and triglycerides during acute phase of experimental infection. C5BL/6 mice were infected with VL-10 of T. cruzi and plasma used to measure total cholesterol (a), HDL cholesterol (b), LDL cholesterol (c), and triglycerides (d) using colorimetric enzymatic kits. Animals were fed with normolipid or high-fat diets and treated or not with simvastatin. White bars: uninfected animals; black bars: animals infected with T. cruzi . The data are mean ± standard error of the mean (SEM).

Article Snippet: The total cholesterol, HDL, and triglycerides were determined in plasma samples, using an enzymatic colorimetric kit for cholesterol, triglycerides, and HDL liquiform, from Labtest Diagnostica SA (Lagoa Santa, MG, BR).

Techniques: Infection, Clinical Proteomics